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clotting time of the 1,25D sample
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le.4444
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 Posted: Fri Dec 18th, 2009 14:03

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In the Physician’s Guide to the Marshall Protocol there are differing directions (refer below) regarding the clotting time of the 1,25D sample.

On page 20 under Special Instructions is the following:

The 1,25D sample should be allowed to settle and clot at room temperature for at least 30 minutes (but no more than two hours) and then centrifuged

On page 5 under Step One is the following:

Remind the drawing lab that the 1,25-D sample must be clotted no more
than 30 minutes before centrifuge

Could you please clarify the processing of the 1,25D sample? Thanks

le.4444
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 Posted: Wed Dec 23rd, 2009 01:38

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I have an appointment with a new physician tomorrow (Wednesday, 12/23) and will be discussing the MP with her. Could someone reply to my question on my previous post (above) regarding the clotting time for the 1,25-D test, in the event that this new physician is willing to order labs for me? Thanks.

edj2001
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 Posted: Wed Dec 23rd, 2009 04:27

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Here are instructions from the MPKB.com:

http://mpkb.org/doku.php/home:tests:dtesting 

Gene 
 

“Doctor's instructions to lab
To facilitate correct handling of the blood sample for 1,25-D and to avoid having to tell the drawing lab how to do their job, patients can ask their doctor to sign and staple the following instructions to the lab order:

Please perform the following Vitamin D tests: 1,25-D and 25-D.
Please ensure that collection staff arrange for centrifuging and freezing of the 1,25D sample. (only)
The sample should be allowed to settle and clot at room temperature for at least 30 minutes (but no more than two hours) and then centrifuged. Do not hold on dry ice prior to centrifuging.
After centrifuging, freeze at between -2 and -10 C.
The sample must be transported on dry ice in order to remain frozen until it reaches the testing lab.
Note that 1,25D levels are exceedingly minute. 1 pg/ml is one millionth part of one millionth part (pico is 10 raised to the -12 power) and the pathology to measure such levels is extremely sensitive to correct handling. For every gm of Vitamin D taken in barely one part in 1,000 ends up as the double hydroxylated”

 



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le.4444
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 Posted: Wed Dec 23rd, 2009 13:48

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Gene,
Thank you for responding so quickly regarding the 1,25-D.

Last edited on Wed Dec 23rd, 2009 14:05 by le.4444


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