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vip.5678
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| Joined: | Fri Sep 5th, 2008 |
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Posted: Mon Apr 6th, 2009 17:46 |
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I have one question.
Once the sample is received frozen in the quest laboratory, testing for vitamin D and 1-25 D should be done in frozen or after thawing.
Is it mandatory that testing be done frozen or after thawing ? or it's ok to do either way. If not, How will the results be affected ?
Sincerely,
Joy.
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JoshR
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| Joined: | Mon Aug 18th, 2008 |
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Posted: Sun Apr 19th, 2009 12:09 |
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I am just guessing here, and have no idea how the assay is done, but I would be surprised if it can be done on a frozen sample. In that case, the ideal would be to do the assay as soon as possible after the sample is thawed.
The longer the sample stays above freezing, the more the 1,25-D level will decay.
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5/6 CFS criteria, 125D36 Ph1Jul08 Ph2Sep08 Ph3Feb09 25D8(Sep08) NoIRs covered up (except hands) low lux home minimal light exp r/t work
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matthew.gatenby
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| Joined: | Wed Feb 4th, 2009 |
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Posted: Thu Apr 23rd, 2009 05:29 |
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Quest actually issued a recall oln vitd testing in january of this year as it was proven their testing method was incorrect.
the are now assuring this test is done correctly.
if your test was taken pre or during january 2008 i would probally ask them about the recall as they will rerun the test cost free.
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Ph1 Nov 08/Mod Ph 2 May09/Phase 2 12Sep/ 25-D 26.4 ng/ml Apr/ DX Lung Sarc 08/08 elevated ALT/Avd vit d/Olmetec 4x/mino 100mg Zith 74mg| ABC of MP|
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matthew.gatenby
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Posted: Thu Apr 23rd, 2009 05:46 |
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Testing for vitd is done via a form of testing called "Liquid Chromatography/Mass Spectrometry/Mass Spectrometry"
the link to the process
http://www.kcl.ac.uk/schools/health/resfac/mspec/instr/lcmsms-intro.html
then once you like me get confused by the above article , learn that
there are several different sample handling methods
atmospheric pressure ionization (API) techniques of electrospray, pneumatically assisted electrospray and atmospheric pressure chemical ionization (APCI) and atmospheric pressure photo ionization (APPI) using single quadrupole, triple quadrupole, time-of-flight and ion trap mass analyzers.
and there is an article about using charcol frozen samples and the acpi/lc/ms/ms
testing methodfor its accuracy by having subjects contolling vitamin d and the test results.
i did not include this article as it has a "pro" perspective on the supplementation of vitd on immune diseases.
however the testing method for all intensive purposes uses a frozen sample and was accurate
and i believe it is thawed at the time it is mixed with the charcol then kept between -70 and -40
if anyone can expand or has more thorough info .. please let us know as i am just going off what i read
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Ph1 Nov 08/Mod Ph 2 May09/Phase 2 12Sep/ 25-D 26.4 ng/ml Apr/ DX Lung Sarc 08/08 elevated ALT/Avd vit d/Olmetec 4x/mino 100mg Zith 74mg| ABC of MP|
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Dr Trevor Marshall
Foundation Staff
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Posted: Thu Apr 23rd, 2009 13:03 |
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Matthew,
The best place to look for info on D-metabolite testing is our Knowledge Base 
http://mpkb.org/doku.php/home:tests:dtesting
http://mpkb.org/doku.php/home:tests:vitdinterpretation
Last edited on Sun Oct 25th, 2009 22:12 by
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john_nz
Member
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Posted: Tue May 26th, 2009 08:57 |
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Hi Matthew
I have just received my 1,25 D result after waiting over two months :
1,25D = 186 no unit of measure supplied
25,D = 54 again no unit of measure supplied
this was done by a Lab in Melbourne - no name supplied
Question : do you think the 1,25 result is authentic ?
I have been on prednisone for six years, when the test was done in March I had just come off antibiotics and my prednisone dose was 20mg per day.
I have now weaned down to 8 per day and am on 40 mg Olmesarten every six hours, I have no idea how to query the lab to see how they handled the sample, but based on what I've read this seems "off the scale " ? I think Io shall have to go back to the lab to see why they would even define the unit of measure ng/ml, or nmol or pg/ml ?
any thoughts?
regards
John
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Sarcoid 2003,MAC 2009, |1-25=186,25D=33 Sept 09| PH1May09 Olmesarten 40x6, totally off pred and inhalers
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matthew.gatenby
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Posted: Tue May 26th, 2009 09:49 |
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John ,
i cannot comment on the medical result
did you use the calculator ..
http://mpkb.org/doku.php/home:tests:dtesting
http://mpkb.org/doku.php/home:tests:vitdinterpretation
put your results into the second link..
you already have a sarc dx so imo ... i wouldnt worry about the 1,25 d result
as it is used for people that are unsure of their dx.
the problem you have is the 25d this from what i know and what the results calc will tell you is it is highly immunosuppressive so .. you will have to really make an effort to get that down.
Last edited on Sun Oct 25th, 2009 22:13 by
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Ph1 Nov 08/Mod Ph 2 May09/Phase 2 12Sep/ 25-D 26.4 ng/ml Apr/ DX Lung Sarc 08/08 elevated ALT/Avd vit d/Olmetec 4x/mino 100mg Zith 74mg| ABC of MP|
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